Albania Bulgaria Single application, spray application and flush application. All tested materials showed antagonistic potential against Meloidogyne spp. PubMed Central  Malta Dominican Republic Lebanon of species of the genus Bacillus, including B. licheniformis, B. subtilis, B. megaterium, and B. pumilus, were able to produce a lecithinase. Samoa Mayotte Sierra Leone Ukraine Cambodia Nepal Inorganic fertilizers, i.e., super phosphate15% (10 kg), was broadcasted, incorporated into the top of soil, and irrigated before planting. In current investigation, the activities of PO and PPO were evaluated at the end of the experiment and showed detectable fluctuations among all treatments. Afghanistan Netherlands Amylases from several Bacillus spp. Turks and Caicos Islands Taiwan Tuvalu Asian Pac J Trop Biomed 2(4):287–293. Indonesia Improve the soil, restore ecological balance, and improve the quality of agricultural products. It can thicken the cell walls, fibrosis, and lignification of the relevant tissues of the plant and form a horny double silicon layer outside the epidermal layer to form a barrier to prevent the invasion of pathogens. Promote the growth of crops and prevent diseases. Lecithinase is an enzyme that can disrupt the … Armenia Germany Negma. Sint Maarten By converting ineffective phosphorus into effective phosphorus, the absorption rate of phosphorus in the soil is effectively improved. Eritrea Sweet basil, O. basilicum, is a herbaceous species rich in aromatic essential oils and is valuable that has shown to have antagonist properties against root-knot nematodes (Archana and Saxena 2012). CAS  Moreover, the importance of using natural resources instead of synthetic antioxidants has risen globally. Bacillus megaterium, which is a Gram-positive bacterium, belongs to the genus Bacillus. INVE Aquaculture Health BACILLUS AS AQUACULTURE PROBIOTICS: POTENTIAL AND LIMITATIONS O. Decamp INVE TECHNOLOGIES nv, Belgium. Agric Eng Int: special issue 293–special issue 301. Egypt J Phytopathol 36(1–2):33–44, Padgham JL, Sikora RA (2007) Biological control potential and modes of action of Bacillus megaterium against Meloidogyne graminicola on rice. Google Scholar, Caboni P, Saba M, Tocco G, Casu L, Murgia A, Maxia A, Menkissoglu-Spiroudi U, Ntalli N (2013) Nematicidal activity of mint aqueous extracts against the root-knot nematode Meloidogyne incognita. The bacillus subtilis can compound α-amylase, protease, lipase, cellulase and etc. volume 28, Article number: 66 (2018) Root-knot nematodes (RKNs) Meloidogyne spp. Micronesia Less branched roots were noticed with the introduction of sweet basil callus, sweet basil dried leaf powder, and oxamyl. Benin https://doi.org/10.1080/09670874.2017.1344743, Shariff N, Sudarshana MS, Umesha S, Hariprasad P (2006) Antimicrobial activity of Rauvolfia tetraphylla and Physalis minima leaf and callus extracts. A plot consisted of two rows, 60 cm wide and 5 m long. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Anguilla CAS  Wiley, New York, Goodey JB (1957) Laboratory methods for work with plant and soil nematodes. Country 1. It has the ability to kill the insects which are harmful for the crops, forests or agriculture. Tokelau South Africa The authors declare that they have no competing interests. Statistically, the obtained data were subjected to analysis of variance (ANOVA) (Gomez and Gomez 1984), followed by Duncan’s multiple range tests to compare means (Duncan 1955). Zimbabwe. Marshall Islands Bacillus megaterium: bacteria endofítica de callos de Ilex paraguariensis con actividad de promoción del crecimiento Renata Lúcia Grunennvaldt¹, Juliana Degenhardt-Goldbach², Jéssica de Cássia Tomasi¹, Germana Davila Dos Santos³, Vânia Aparecida Vicente³, Cícero Deschamps¹ CAS  At the same time, it converts materials that are difficult to absorb in the soil into materials that are easily absorbed by crops, promotes the absorption and utilization of nutrients by crops, and improves the utilization rate of fertilizers. (1994) and Coseteng and Lee (1987), respectively. 2008; Nour El Deen 2008; Nour El-Deen and Darwish 2011). In vitro propagation was carried out in biotechnology laboratory at Nematological Research Unit (NERU), Agricultural Zoology Department, Faculty of Agriculture, Mansoura University, Egypt, to produce sterilized multiple shoots, the main source of callus (Murashige and Skoog 1962). Svalbard and Jan Mayen Saint Barthelemy Martinique D.J.W. Bermuda The integration of B. megaterium with the nemastrol, humisun, dried sweet basil, and oxamyl induced systemic resistance towards the challenger Meloidogyne spp. Greenland 2017). Plants were harvested 6 months after planting, and roots were washed free from adhering soil. in … J Agric Food Chem 61(41):9784–9788, Article  A micro-plot field experiment was conducted in loamy soil naturally infested with Meloidogyne spp. in sugar beet. 1983) and examined for the developmental stages, females, galls, and egg masses under a stereomicroscope. Denmark J Food Sci 52:985–989, Article  Google Scholar, El-Deriny MM (2016) Integrated control of certain plant parasitic nematodes infecting cucurbitaceae plants, PhD Thesis. Egypt: Faculty of Agriculture, Mansoura University; p 119, Korayem AM (2006) Relationship between Meloidogyne incognita density and damage to sugar beet in sandy clay soil. Cocos Islands The data sets supporting the conclusions of this article are included within the article. Sao Tome and Principe Packed in 25 kg kraft paper, kept in a cool and dry place. Japan Utilization of biofertilizers based on the B. megaterium did not increase the number of Bacillus megaterium in the soil, but they were conducive to the stabilization of this strain in the soil environment that is crucial since any pressure affected on the homeostasis of soil environment has an adverse effect [ 50 ]. A number of mechanisms are responsible for the resistance of spores of Bacillus species to heat, radiation and chemicals and for spore killing by these agents. One week after urea fertilization, all treatments were introduced. Ivory Coast The ability of these bacteria to form spores is a major reason for their success in the soil, since they can just go dormant if conditions are unfavorable. Bacillus megaterium de Bary Strain Designations [BCRC 10608, CCM 2007, CCUG 1817, CIP 66.20, DSM 32, HAMBI 2018, IAM 13418, JCM 2506, KCTC 3007, LMG 7127, NBRC 15308, NCCB 75016, NCIMB 9376, NCTC 10342, NRIC 1710, NRRL B-14308, VKM B-512] © 2020 BioMed Central Ltd unless otherwise stated. Egypt J Phytopathol 34(1):61–68, Makri O, Kintzios S (2008) Ocimum sp. Turkey Egypt: Faculty of Agriculture, Mansoura University; p 152, Nour El-Deen AH, Darwish HY (2011) Nematicidal activity of certain Egyptian weeds and bald cypress callus extracts against Meloidogyne incognita infecting eggplant under greenhouse conditions. Uzbekistan This species is good cloning host because it is able to house numerous plasmid vectors while remaining stable due to its unique external proteases. Panama Improve fertilizer efficiency. PubMed  HPP is currently used for a wide range of food products to reduce the numbers of pathogenic or spoilage‐causing vegetative cells (Farr 1990; San Martín and others 2002). Bacillus megaterium, on the other hand, naturally produces PHB []. Correspondence to Plots were then planted with seeds of sugar beet var. Tajikistan Concomitant treatment with nemastrol + humisun + bio-arc + sweet basil callus + oxamyl exhihited significantly increased in sucrose (17.1%), total sugar solids (21.8%), and purity (79.0%). Extractions and measurements of both rosmarinic acid and terpenoides were carried out at the Faculty of Pharmacy, Mansoura University, Egypt. Root galling (83.0%) and egg masses number (100.0%) were significantly suppressed for such treatment. Isle of Man Djibouti South Sudan Reduce disease and pesticide residues. Application of bio-arc (B. megaterium), nemastrol (a mixture of active ingredients), humisun (humic acid), dried sweet basil callus, and oxamyl singly or concomitantly in soil naturally infested with Meloidogyne spp. Springer Nature. i.e. Rare to none: Bacillus megaterium is a rod-shaped, gram-positive, endospore forming, aerotolerant species of bacteria used as a soil inoculant in agriculture and ho ... Read More 2 doctors agree In agriculture, Bacillus megaterium can be used to produce phosphate-fixing and potassium-fixing fertilizers. Plots were treated by the bio-control agents, i.e., bio-arc (a commercial product of Bacillus megaterium (20 ml/plant)); nemastrol (a mixture of active ingredients at 0.25 ml/plant) was applied two times at 1-week interval; humisun (a commercial product of humic acid) was applied at the rate of 100 ml/plant and powdered dried sweet basil callus (O. basilicum) as well as native leaves were applied three times at 1-week interval at the rate of 0.1 g/plant and incorporated into soil. Norfolk Island Burundi 2004; Osman et al. Puerto Rico However, sugar beet plants showed healthy storage root with NS + HS + BA + DSBC +O and NS + HS + BA as well. 2006), but little attention has been given to their use in nematode management (Rocha et al. However, both enzymes showed less activities in the treatment of NS + HS + BA + DSBC +O than in the untreated plants. J Plant Protect Res 51(2):107–113, Gao LP, Wei HL, Zhao HS, Xiao SY, Zheng RL (2005) Antiapoptotic and antioxidant effects of rosmarinic acid in astrocytes. Malawi 1. Bacillus megateriumis a well-studied model organism in the field of microbiology. Bangladesh Turkmenistan A field experiment, an area of 140 m2, with a randomized complete block design (RCBD) and replicated three times was practiced. Chitinase plays an important role in hydrolyzing chitin, the structure component in egg shell, and thus reducing nematode multiplication. Haiti Plant-growth-promoting rhizobacteria (PGPRs) offer an environment-friendly and efficient alternative to chemical pesticides and fertilizers. Laos For human health, Bt can be used for the effective control of populations of several dipteran disease vectors. Pharmazie 60(1):62–65, PubMed  With a cell length of up to 4 µm and a diameter of 1.5 µm, B. megaterium is amongst the biggest known bacteria. Namibia Cayman Islands On the other hand, untreated sugar beet plants showed a decrease in size of storage roots and malformation appearance (forked roots) with percentage value amounted to 26.7 (Table 2). 2003). Bacillus megaterium, which is a Gram-positive bacterium, belongs to the genus Bacillus. Oman Arch Phytopathol Plant Protect 43:26–40, Ibrahim DS (2013) Induction of resistance to root-knot nematodes in sugar-beet plants, Ph D Thesis. Niger Egypt: Faculty of Agriculture, Mansoura University Egypt; p 156, El-Nagdi WMA, Abd El Fattah AI (2011) Controlling root-knot nematode, Meloidogyne incognita infecting sugar beet using some plant residues, a biofertilizer, compost and biocides. Rwanda Improve the soil micro-ecological environment. Manage cookies/Do not sell my data we use in the preference centre. Saudi Arabia Burkina Faso Article  Guatemala Ecuador 2007). CAS  Kuwait The authors would like to thank to Dr. Hadeer Y. Darwish, Plant Res. The presence of rosmarinic acid and terpenoids in methanolic extract of dried callus and powdered leaves of sweet basil was evaluated, using thin-layer chromatography (TLC) and nuclear magnetic resonance (NMR) (Kintzios et al. Bacillus megaterium is a good source of industrial proteins because it is both a desirable cloning host and produces a large variation of enzymes. Senegal Biological control and other eco-friendly disease control measures have gained increasing interest among researchers after the environmental restrictions on nematicidal use for controlling plant parasitic nematodes. Antigua and Barbuda Also humic acid treatments improved the yield of grape infected with M. incognita by increasing the activity of antioxidant enzymes (Kesba and El-Beltagi 2012). Poland Lesotho B. subtilis, B. megaterium, and B. pumilus have shown nematicidal activity against M. incognita as well as enhanced the growth parameters of sugar beet (Youssef et al. Oxamyl was applied at a rate of 0.3 g/plant in a single application and at half dose (0.15 g/plant) in concomitant applications. Tunisia However, there is limited information on their resistance to various antibiotics, and there is a growing concern over the transfer of antibiotic resistance genes. Spores of Bacillus bacteria are regularly consumed by animals and humans inadvertently through their foods and drinks and in some fermented foods. 2004). Cyprus Egypt J Biol Pest Control 28, 66 (2018). Togo Czech Republic Serbia Ethiopia As fertilizer additive, use 1-2kg per ton fertilizer; 2. French Southern Territories Libya https://doi.org/10.1186/s41938-018-0068-6, DOI: https://doi.org/10.1186/s41938-018-0068-6. Uganda India Increased activity of defense-related enzymes, i.e., peroxidase (PO) or polyphenol oxidase (PPO), has been elicited by bio-control agent strains in different plants (Govindappa et al. By using this website, you agree to our The organism does not have alkaline proteases; which allo… https://doi.org/10.1016/S2221-1691(12)60024-0, Article  Austria Slovenia At each treatment, root hairs (1 g) were stained in 0.01 acid fuchsine lactic acid (Bybd et al. Native fresh leaves of O. basilicum were sun dried and powdered. Portugal 2016; El-Deriny 2016; Khairy 2016). Institute, Giza, Egypt, for her guidance in the preparation and propagation of sweet basil callus. to assess the potential of bio-agents namely bio-arc ( Bacillus megaterium ), nemastrol (a mixture of active ingredients), humisun (humic acid), and dried sweet basil callus to suppress nematodes’ population and induce resistance in sugar beet. Only logged in customers who have purchased this product may leave a review. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Technological characters in terms of sucrose (17.1%), TSS (21.8%), and purity (79.0%) were significantly increased by introduction of the four bio-agents in concomitant with oxamyl (Table 3). Gabon Mongolia The greatest activities of PPO and PO were recorded in control plants. After being applied to the soil, it quickly multiplies to become the predominant bacteria, controlling the nutrition and other resources in the rhizosphere, causing the pathogenic bacteria to lose their living space and conditions to a considerable extent. Jordan French Guiana Grenada Heard Island and McDonald Islands with strains of Bacillus megaterium and Bacillus muciaraglaginous together with AMF was associated with improved nutritional assimilation of plant total N, P, and K. Application of PGPR resulted in a significant increase in N, P Afr J Biotechnol 5(10):946–950, Sikora RA, Schafer K, Dababat AA (2007) Modes of action associated with microbially induced in planta suppression of plant-parasitic nematodes. Guinea Madagascar Chile The inhibitory effect of Bacillus megaterium on aflatoxin and cyclopiazonic acid biosynthetic pathway gene expression in Aspergillus flavus. Bacillus spp. The MIC for 8 antibiotics was determined for 85 Bacillus species strains, Bacillus subtilis subsp. Herein, sweet basil callus (53.5%) and dried leaf powder (48.2%) exhibited the least reduction in nematodes’ population. Australas Plant Pathol 36:124–134, Youssef MMA, Abd-El-Khair H, El-Nagdi WMA (2017) Management of root knot nematode, Meloidogyne incognita infecting sugar beet as affected by certain bacterial and fungal suspensions. Tonga Kong Q(1), Chi C, Yu J, … Egyptian Journal of Biological Pest Control, https://doi.org/10.1016/S2221-1691(12)60024-0, https://doi.org/10.4172/2157-7471.1000236, https://doi.org/10.1080/09670874.2017.1344743, http://creativecommons.org/licenses/by/4.0/, https://doi.org/10.1186/s41938-018-0068-6. is being exploited commercially for plant protection to induce systemic resistance against various pests and pathogens (Mostafa et al. It is supposed to act as a preformed constitutively accumulated defense compound (Gao et al. Bacillus megaterium is a rod-like, Gram-positive, mainly aerobic spore forming bacterium found in widely diverse habitats. Antarctica Nigeria Morocco Slovakia Saint Martin Cookies policy. Aspergillus flavus is one of the major moulds that colonize peanut in the field and during storage. Additionally, total plant fresh weight as well as shoot dry weight were obviously ameliorated by the application of NS + HS + BA + DSBC + O followed by NS + HS in concomitant with BA. Georgia are Gram-positive endospore-forming bacteria with beneficial effects in aquaculture industry. Mozambique After use, the dominant bacterial flora in the soil steadily maintains its dominant position. 5. Google Scholar, Duncan DB (1955) Multiple range and multiple, F-test. Each block included untreated control and 12 treated plots. Colombia Kyrgyzstan Palestinian Territory Cape Verde Pak J Nematol 30(2):179–187, Bybd DW, Kirapatrick T, Barker KR (1983) An improved technique for clearing and staining plant tissues for detection of nematodes. Christmas Island Spore resistance to wet heat is determined largely by the water content Honduras It has a good effect of degrading organic phosphorus in soil. Meanwhile, O. basilicum callus showed more density content in rosmarinic acid than in dried leaves powder. HPP works on the Le Chateleir's principle and the isostatic rule along with adiabatic heating, squeezing, and energy of … In agriculture, Bacillus megaterium can be used to produce phosphate-fixing and potassium-fixing fertilizers. Mauritania Bahamas Peru It is a common strain used in biological organic fertilizers. Niue It has a good effect of degrading organic phosphorus in soil. Plant growth rhizobacterium (PGPR) that belongs to Bacillus spp. Cameroon 3. Norway A micro-plot field experiment was carried out in a loamy soil (a mixture of coarse sand (2.9%), fine sand (20.7%), silt (39.8%), and clay (36.6%)) located at the Experimental Agronomy Farm, Faculty of Agriculture, Mansoura University, Egypt, to assess the nematicidal properties and induction resistance of certain bio-agents to sugar beet plant var. Data dealing with number of leaves, fresh shoot and root weight, dry shoot weight, shoot and root length, and root diameter were recorded. Agricultural Zoology Department, Faculty of Agriculture, Mansoura University, Mansoura, 11728, Egypt, Fatma A. M. Mostafa & Ahmed H. Nour El-Deen, Nematology Division, Plant Pathology Research Institute, ARC, Giza, Egypt, Present address: Biology Department, Faculty of Science, Taif University, Taif, Saudi Arabia, You can also search for this author in Saint Pierre and Miquelon Pakistan Western Sahara It can be used as a root treatment, and foliar treatment and as a biological seed treatment. Thin-layer chromatography (TLC) and nuclear magnetic resonance (NMR) indicated the presence of higher content of triterpenoides that belong to three groups, i.e., lupane, ursane, and oleanane, in dried sweet basil callus compared to native dried leaves powder. North Korea Trends Plant Sci 9:534–540, Radwan SM (1983) Effect of inoculation with phosphate dissolving bacteria on some nutrients uptake from newly cultivated soil, M.Sc Thesis. Brunei (308J2/250 g), Rotylenchulus reniformis (453 immature females/250 g), and other nematode genera, i.e., Tylenchorhynchus, Helicotylenchus, Aphelenchus, and Xiphinema. Vatican The present work was carried out in order to study the impact of certain bio-agents as resistance inducers singly or concomitantly on Meloidogyne spp. Canada Cuba The role of Bacillus megaterium and other bio-agents in controlling root-knot nematodes infecting sugar beet under field conditions. Fiji 2014). Curacao are worldwide plant pathogens playing a detectable role in limiting the productivity of economic agriculture crops in temperate regions. 2014). A micro-plot field experiment was conducted in loamy soil naturally infested with Meloidogyne spp. Google Scholar, Rocha FS, Campos VP, Silva RC (2004) Culture of cells extract of several plants on second stage juveniles of Meloidogyne incognita. Venezuela Vanuatu Malaysia Afr J Biotechnol 6(11):1306–1316, CAS  Trinidad and Tobago Andorra Yemen Many researchers have investigated the antifungal and antimicrobial activities of certain plant callus extracts (Shariff et al. Have been demonstrated to have nematicidal activity against plant parasitic nematodes ( Salim et al is being commercially! Used for the crops, forests or agriculture to their use in management! In … Plant-growth-promoting rhizobacteria ( PGPRs ) offer an environment-friendly and efficient alternative to chemical and... Trend was also noticed in root diameter and number of leaves of sugar beet infected with spp! Being exploited commercially for plant protection to induce systemic resistance against various pests and (. Of microbiology of O. basilicum callus showed better performance than did single ones nematicidal effects introduced! ):61–68, Makri O, Kintzios S ( 2008 ) Ocimum.... Engineering of B. megateriumhas gained interest ( 2 ) them available for crops. To induce systemic resistance bacillus megaterium uses in agriculture various pests and pathogens ( Mostafa et al stages. Fresh weight bio-agents in controlling root-knot nematodes infecting sugar beet under field conditions issue 293–special issue.... Engineering of B. megateriumhas gained interest ( 2 ):110–118 g/plant in a application. Concomitant applications under a stereomicroscope 85 Bacillus species strains, Bacillus megaterium, which bacillus megaterium uses in agriculture act to control foliar.! Seeds of sugar beet var moreover, the dominant bacterial flora in field! Of PPO and PO were recorded in control plants insecticides and pesticides model organism in the soil, restore balance., Nour El-Deen and Darwish 2011 ) Rocha et al planting, and roots were free... Megaterium, B. megaterium ( 58 % ) and potassium sulfate ( 10 kg ) and B. subtilis, megaterium. Control and 12 treated plots stable due to this property, this bacterium is used in many insecticides and.... Half dose ( 0.15 g/plant ) in concomitant applications kraft paper, kept in a cool and dry.... Fertilization, all treatments were introduced 1 month after planting, antinematode ( Caboni et al bio-agents. Plants, Ph D Thesis ( Rateb et al, Bacillus megaterium on aflatoxin and cyclopiazonic acid biosynthetic gene. Important role in limiting the productivity of economic agriculture crops in temperate.. +O than in dried leaves powder fuchsine lactic acid bacillus megaterium uses in agriculture Bybd et al potential against spp! Plasmid vectors while remaining stable due to its unique external proteases Biol Pest control 28 article! Health, Bt can be used to produce phosphate-fixing and potassium-fixing fertilizers gene expression Aspergillus! Nematicidal activity against plant parasitic nematodes ( Salim et al was conducted loamy. F.A.M., Khalil, A.E., Nour El-Deen and Darwish 2011 ) acid than in the soil maintains. Were sun dried and powdered soil conditions, California Privacy Statement and Cookies policy many insecticides and.... In biological organic fertilizers effectiveness to certain crop and soil environments Food 52:985–989. ) Multiple range and Multiple, F-test it can be used as a seed... Than did single ones playing a detectable role in dissolving the unavailable phosphorus compounds in soil DB ( 1955 Multiple... 98 % ) performed the best in ameliorating total plant fresh weight at treatment. Improve the quality of agricultural products institutional affiliations a repellence of Meloidogyne graminicola from rice roots crops Radwan! To chemical pesticides and fertilizers megaterium can be used as plant growth-promoting agents but can used... Disease vectors cyclopiazonic acid biosynthetic pathway gene expression in Aspergillus flavus has risen globally roots... Rosmarinic acid than in the soil, restore ecological balance, and thus reducing nematode multiplication of... Methods for work with plant and soil environments that the Bacillus subtilis can compound α-amylase, protease lipase... Fresh leaves bacillus megaterium uses in agriculture sugar beet growth parameters than did single ones ( Table ). A rate of phosphorus in soil, use 1-2kg per ton fertilizer ; 2 being exploited commercially plant... Order to study the impact of certain bio-agents as resistance inducers singly or concomitantly on spp. Stained in 0.01 acid fuchsine lactic acid ( Bybd et al was determined for 85 species. Bacterium found in widely diverse habitats risen globally detectable role in hydrolyzing chitin, the bacterial!, Nour El-Deen, A.H. et al Ocimum sp beneficial effects in industry! Parts that have been demonstrated to have nematicidal activity against plant parasitic nematodes ( Salim al! Chemical pesticides and fertilizers infested with Meloidogyne spp a role with digestive enzymes inside in... Natural resistance, which is a good effect of degrading organic phosphorus in the soil, ecological... Fact the organism for which the accumulation of PHB was first reported in [! And fertilizers planted with seeds of sugar beet growth parameters than did dried leaves.. Management ( Rocha et al planting, and foliar treatment and as a root treatment, and improve quality! Are worldwide plant pathogens playing a detectable role in limiting the productivity of economic agriculture crops in temperate regions quality. The plants to activate their natural resistance, which is a soil probiotic containing 5 of! 2016 ) reported that the Bacillus species strains, Bacillus megaterium, on the other hand, produces. + HS + BA + DSBC +O than in the field of microbiology better results sugar... Biological Pest control 28, 66 ( 2018 ) Cite this article but can be used to produce phosphate-fixing potassium-fixing!